Liquid Fungal Cocultivation as a Strategy to Access Bioactive Metabolites.
Identifieur interne : 000132 ( Main/Exploration ); précédent : 000131; suivant : 000133Liquid Fungal Cocultivation as a Strategy to Access Bioactive Metabolites.
Auteurs : Airton Damasceno Silva [Brésil] ; Alessandra Regina Pepe Ambrozin [Brésil] ; Ana Flávia S. De Camargo [Brésil] ; Felipe De Paula Nogueira Cruz [Brésil] ; Leonardo Luiz Gomes Ferreira [Brésil] ; Renata Krogh [Brésil] ; Taynara Lopes Silva [Brésil] ; Ilana Lopes Baratella Da Cunha Camargo [Brésil] ; Adriano Defini Andricopulo [Brésil] ; Paulo Cezar Vieira [Brésil]Source :
- Planta medica [ 1439-0221 ] ; 2020.
Abstract
Fungi are a rich source of bioactive compounds. Fungal cocultivation is a method of potentiating chemical interactions and, consequently, increasing bioactive molecule production. In this study, we evaluated the bactericidal, antiprotozoal, and cathepsin V inhibition activities of extracts from axenic cultures of 6 fungi (Fusarium guttiforme, Pestalotiopsis diospyri, Phoma caricae-papayae, Colletotrichum horii, Phytophthora palmivora, and C. gloeosporioides) that infest tropical fruits and 57 extracts obtained by their cocultivation. Our results reveal that fungal cocultivation enhances the biological activity of the samples, since all extracts that were active on Gram-positive bacteria, Gram-negative bacteria, Trypanosoma cruzi, and Leishmania infantum were obtained from cocultivation. Bacterial growth is either totally or partially inhibited by 46% of the extracts. Two extracts containing mainly fusaric and 9,10-dehydrofusaric acids were particularly active. The presence of the fungus F. guttiforme in co-cultures that give rise to extracts with the highest activities against L. infantum. An axenic culture gave rise to the most active extract for the inhibition of cathepsin V; however, other coculture extracts also exhibited activity toward this biological target. Therefore, the results of the biological activities indicate that fungal cocultivation increased the biological potential of samples, likely due to the hostile and competitive environment that pushes microorganisms to produce substances important for defense and allows access to metabolic routes then silenced in milder cultivation conditions.
DOI: 10.1055/a-1200-2046
PubMed: 32645738
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type="university">Université de São Paulo</orgName></affiliation></author><author><name sortKey="Andricopulo, Adriano Defini" sort="Andricopulo, Adriano Defini" uniqKey="Andricopulo A" first="Adriano Defini" last="Andricopulo">Adriano Defini Andricopulo</name><affiliation wicri:level="4"><nlm:affiliation>São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP</wicri:regionArea><placeName><region type="state">État de São Paulo</region><settlement type="city">São Paulo</settlement></placeName><orgName type="university">Université de São Paulo</orgName></affiliation></author><author><name sortKey="Vieira, Paulo Cezar" sort="Vieira, Paulo Cezar" uniqKey="Vieira P" first="Paulo Cezar" last="Vieira">Paulo Cezar Vieira</name><affiliation wicri:level="2"><nlm:affiliation>Department of Chemistry, Federal 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Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Chemistry, Federal University of São Carlos (UFSCar), São Carlos, SP</wicri:regionArea><placeName><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Ambrozin, Alessandra Regina Pepe" sort="Ambrozin, Alessandra Regina Pepe" uniqKey="Ambrozin A" first="Alessandra Regina Pepe" last="Ambrozin">Alessandra Regina Pepe Ambrozin</name><affiliation wicri:level="2"><nlm:affiliation>Institute of Science and Technology, Federal University of Alfenas (Unifal-MG), Poços de Caldas, MG, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Institute of Science and Technology, Federal University of Alfenas (Unifal-MG), Poços de Caldas, MG</wicri:regionArea><placeName><region type="state">Minas Gerais</region></placeName></affiliation></author><author><name sortKey="De Camargo, Ana Flavia S" sort="De Camargo, Ana Flavia S" uniqKey="De Camargo A" first="Ana Flávia S" last="De Camargo">Ana Flávia S. De Camargo</name><affiliation wicri:level="4"><nlm:affiliation>São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP</wicri:regionArea><placeName><region type="state">État de São Paulo</region><settlement type="city">São Paulo</settlement></placeName><orgName type="university">Université de São Paulo</orgName></affiliation></author><author><name sortKey="Cruz, Felipe De Paula Nogueira" sort="Cruz, Felipe De Paula Nogueira" uniqKey="Cruz F" first="Felipe De Paula Nogueira" last="Cruz">Felipe De Paula Nogueira Cruz</name><affiliation wicri:level="4"><nlm:affiliation>São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP</wicri:regionArea><placeName><region type="state">État de São Paulo</region><settlement type="city">São Paulo</settlement></placeName><orgName type="university">Université de São Paulo</orgName></affiliation></author><author><name sortKey="Ferreira, Leonardo Luiz Gomes" sort="Ferreira, Leonardo Luiz Gomes" uniqKey="Ferreira L" first="Leonardo Luiz Gomes" last="Ferreira">Leonardo Luiz Gomes Ferreira</name><affiliation wicri:level="4"><nlm:affiliation>São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP</wicri:regionArea><placeName><region type="state">État de São Paulo</region><settlement type="city">São Paulo</settlement></placeName><orgName type="university">Université de São Paulo</orgName></affiliation></author><author><name sortKey="Krogh, Renata" sort="Krogh, Renata" uniqKey="Krogh R" first="Renata" last="Krogh">Renata Krogh</name><affiliation wicri:level="4"><nlm:affiliation>São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP</wicri:regionArea><placeName><region type="state">État de São Paulo</region><settlement type="city">São Paulo</settlement></placeName><orgName type="university">Université de São Paulo</orgName></affiliation></author><author><name sortKey="Silva, Taynara Lopes" sort="Silva, Taynara Lopes" uniqKey="Silva T" first="Taynara Lopes" last="Silva">Taynara Lopes Silva</name><affiliation wicri:level="4"><nlm:affiliation>Department of BioMolecular Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of BioMolecular Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP</wicri:regionArea><placeName><region type="state">État de São Paulo</region><settlement type="city">São Paulo</settlement></placeName><orgName type="university">Université de São Paulo</orgName></affiliation></author><author><name sortKey="Camargo, Ilana Lopes Baratella Da Cunha" sort="Camargo, Ilana Lopes Baratella Da Cunha" uniqKey="Camargo I" first="Ilana Lopes Baratella Da Cunha" last="Camargo">Ilana Lopes Baratella Da Cunha Camargo</name><affiliation wicri:level="4"><nlm:affiliation>São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP</wicri:regionArea><placeName><region type="state">État de São Paulo</region><settlement type="city">São Paulo</settlement></placeName><orgName type="university">Université de São Paulo</orgName></affiliation></author><author><name sortKey="Andricopulo, Adriano Defini" sort="Andricopulo, Adriano Defini" uniqKey="Andricopulo A" first="Adriano Defini" last="Andricopulo">Adriano Defini Andricopulo</name><affiliation wicri:level="4"><nlm:affiliation>São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP</wicri:regionArea><placeName><region type="state">État de São Paulo</region><settlement type="city">São Paulo</settlement></placeName><orgName type="university">Université de São Paulo</orgName></affiliation></author><author><name sortKey="Vieira, Paulo Cezar" sort="Vieira, Paulo Cezar" uniqKey="Vieira P" first="Paulo Cezar" last="Vieira">Paulo Cezar Vieira</name><affiliation wicri:level="2"><nlm:affiliation>Department of Chemistry, Federal University of São Carlos (UFSCar), São Carlos, SP, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Chemistry, Federal University of São Carlos (UFSCar), São Carlos, SP</wicri:regionArea><placeName><region type="state">État de São Paulo</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Department of BioMolecular Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of BioMolecular Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP</wicri:regionArea><placeName><region type="state">État de São Paulo</region><settlement type="city">São Paulo</settlement></placeName><orgName type="university">Université de São Paulo</orgName></affiliation></author></analytic><series><title level="j">Planta medica</title><idno type="eISSN">1439-0221</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Fungi are a rich source of bioactive compounds. Fungal cocultivation is a method of potentiating chemical interactions and, consequently, increasing bioactive molecule production. In this study, we evaluated the bactericidal, antiprotozoal, and cathepsin V inhibition activities of extracts from axenic cultures of 6 fungi (<i>Fusarium guttiforme, Pestalotiopsis diospyri, Phoma caricae-papayae, Colletotrichum horii, Phytophthora palmivora</i>, and <i>C. gloeosporioides</i>) that infest tropical fruits and 57 extracts obtained by their cocultivation. Our results reveal that fungal cocultivation enhances the biological activity of the samples, since all extracts that were active on Gram-positive bacteria, Gram-negative bacteria, <i>Trypanosoma cruzi</i>, and <i>Leishmania infantum</i> were obtained from cocultivation. Bacterial growth is either totally or partially inhibited by 46% of the extracts. Two extracts containing mainly fusaric and 9,10-dehydrofusaric acids were particularly active. The presence of the fungus <i>F. guttiforme</i> in co-cultures that give rise to extracts with the highest activities against <i>L. infantum</i>. An axenic culture gave rise to the most active extract for the inhibition of cathepsin V; however, other coculture extracts also exhibited activity toward this biological target. Therefore, the results of the biological activities indicate that fungal cocultivation increased the biological potential of samples, likely due to the hostile and competitive environment that pushes microorganisms to produce substances important for defense and allows access to metabolic routes then silenced in milder cultivation conditions.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">32645738</PMID><DateRevised><Year>2020</Year><Month>07</Month><Day>09</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1439-0221</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>Jul</Month><Day>09</Day></PubDate></JournalIssue><Title>Planta medica</Title><ISOAbbreviation>Planta Med</ISOAbbreviation></Journal><ArticleTitle>Liquid Fungal Cocultivation as a Strategy to Access Bioactive Metabolites.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.1055/a-1200-2046</ELocationID><Abstract><AbstractText>Fungi are a rich source of bioactive compounds. Fungal cocultivation is a method of potentiating chemical interactions and, consequently, increasing bioactive molecule production. In this study, we evaluated the bactericidal, antiprotozoal, and cathepsin V inhibition activities of extracts from axenic cultures of 6 fungi (<i>Fusarium guttiforme, Pestalotiopsis diospyri, Phoma caricae-papayae, Colletotrichum horii, Phytophthora palmivora</i>, and <i>C. gloeosporioides</i>) that infest tropical fruits and 57 extracts obtained by their cocultivation. Our results reveal that fungal cocultivation enhances the biological activity of the samples, since all extracts that were active on Gram-positive bacteria, Gram-negative bacteria, <i>Trypanosoma cruzi</i>, and <i>Leishmania infantum</i> were obtained from cocultivation. Bacterial growth is either totally or partially inhibited by 46% of the extracts. Two extracts containing mainly fusaric and 9,10-dehydrofusaric acids were particularly active. The presence of the fungus <i>F. guttiforme</i> in co-cultures that give rise to extracts with the highest activities against <i>L. infantum</i>. An axenic culture gave rise to the most active extract for the inhibition of cathepsin V; however, other coculture extracts also exhibited activity toward this biological target. Therefore, the results of the biological activities indicate that fungal cocultivation increased the biological potential of samples, likely due to the hostile and competitive environment that pushes microorganisms to produce substances important for defense and allows access to metabolic routes then silenced in milder cultivation conditions.</AbstractText><CopyrightInformation>Georg Thieme Verlag KG Stuttgart · New York.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y" EqualContrib="N"><LastName>Silva</LastName><ForeName>Airton Damasceno</ForeName><Initials>AD</Initials><AffiliationInfo><Affiliation>Department of Chemistry, Federal University of São Carlos (UFSCar), São Carlos, SP, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y" EqualContrib="N"><LastName>Ambrozin</LastName><ForeName>Alessandra Regina Pepe</ForeName><Initials>ARP</Initials><AffiliationInfo><Affiliation>Institute of Science and Technology, Federal University of Alfenas (Unifal-MG), Poços de Caldas, MG, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y" EqualContrib="N"><LastName>de Camargo</LastName><ForeName>Ana Flávia S</ForeName><Initials>AFS</Initials><AffiliationInfo><Affiliation>São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y" EqualContrib="N"><LastName>Cruz</LastName><ForeName>Felipe De Paula Nogueira</ForeName><Initials>FPN</Initials><AffiliationInfo><Affiliation>São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y" EqualContrib="N"><LastName>Ferreira</LastName><ForeName>Leonardo Luiz Gomes</ForeName><Initials>LLG</Initials><AffiliationInfo><Affiliation>São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y" EqualContrib="N"><LastName>Krogh</LastName><ForeName>Renata</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y" EqualContrib="N"><LastName>Silva</LastName><ForeName>Taynara Lopes</ForeName><Initials>TL</Initials><AffiliationInfo><Affiliation>Department of BioMolecular Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y" EqualContrib="N"><LastName>Camargo</LastName><ForeName>Ilana Lopes Baratella da Cunha</ForeName><Initials>ILBDC</Initials><AffiliationInfo><Affiliation>São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y" EqualContrib="N"><LastName>Andricopulo</LastName><ForeName>Adriano Defini</ForeName><Initials>AD</Initials><AffiliationInfo><Affiliation>São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y" EqualContrib="N"><LastName>Vieira</LastName><ForeName>Paulo Cezar</ForeName><Initials>PC</Initials><Identifier Source="ORCID">0000-0003-4207-6217</Identifier><AffiliationInfo><Affiliation>Department of Chemistry, Federal University of São Carlos (UFSCar), São Carlos, SP, Brazil.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of BioMolecular Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>Fundação de Amparo à Pesquisa do Estado de São Paulo</GrantID><Agency>#13/07600-3</Agency><Country></Country></Grant><Grant><GrantID>Fundação de Amparo à Pesquisa do Estado de São Paulo</GrantID><Agency>#14/50249-8</Agency><Country></Country></Grant><Grant><GrantID>Fundação de Amparo à Pesquisa do Estado de São Paulo</GrantID><Agency>#2019/04788-8</Agency><Country></Country></Grant><Grant><GrantID>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior</GrantID><Agency>Finance Dode 001</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>07</Month><Day>09</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Planta Med</MedlineTA><NlmUniqueID>0066751</NlmUniqueID><ISSNLinking>0032-0943</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><CoiStatement>The authors declare that they have no conflict of interest.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>7</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>7</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>7</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32645738</ArticleId><ArticleId IdType="doi">10.1055/a-1200-2046</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Brésil</li></country><region><li>Minas Gerais</li><li>État de São Paulo</li></region><settlement><li>São Paulo</li></settlement><orgName><li>Université de São Paulo</li></orgName></list><tree><country name="Brésil"><region name="État de São Paulo"><name sortKey="Silva, Airton Damasceno" sort="Silva, Airton Damasceno" uniqKey="Silva A" first="Airton Damasceno" last="Silva">Airton Damasceno Silva</name></region><name sortKey="Ambrozin, Alessandra Regina Pepe" sort="Ambrozin, Alessandra Regina Pepe" uniqKey="Ambrozin A" first="Alessandra Regina Pepe" last="Ambrozin">Alessandra Regina Pepe Ambrozin</name><name sortKey="Andricopulo, Adriano Defini" sort="Andricopulo, Adriano Defini" uniqKey="Andricopulo A" first="Adriano Defini" last="Andricopulo">Adriano Defini Andricopulo</name><name sortKey="Camargo, Ilana Lopes Baratella Da Cunha" sort="Camargo, Ilana Lopes Baratella Da Cunha" uniqKey="Camargo I" first="Ilana Lopes Baratella Da Cunha" last="Camargo">Ilana Lopes Baratella Da Cunha Camargo</name><name sortKey="Cruz, Felipe De Paula Nogueira" sort="Cruz, Felipe De Paula Nogueira" uniqKey="Cruz F" first="Felipe De Paula Nogueira" last="Cruz">Felipe De Paula Nogueira Cruz</name><name sortKey="De Camargo, Ana Flavia S" sort="De Camargo, Ana Flavia S" uniqKey="De Camargo A" first="Ana Flávia S" last="De Camargo">Ana Flávia S. De Camargo</name><name sortKey="Ferreira, Leonardo Luiz Gomes" sort="Ferreira, Leonardo Luiz Gomes" uniqKey="Ferreira L" first="Leonardo Luiz Gomes" last="Ferreira">Leonardo Luiz Gomes Ferreira</name><name sortKey="Krogh, Renata" sort="Krogh, Renata" uniqKey="Krogh R" first="Renata" last="Krogh">Renata Krogh</name><name sortKey="Silva, Taynara Lopes" sort="Silva, Taynara Lopes" uniqKey="Silva T" first="Taynara Lopes" last="Silva">Taynara Lopes Silva</name><name sortKey="Vieira, Paulo Cezar" sort="Vieira, Paulo Cezar" uniqKey="Vieira P" first="Paulo Cezar" last="Vieira">Paulo Cezar Vieira</name><name sortKey="Vieira, Paulo Cezar" sort="Vieira, Paulo Cezar" uniqKey="Vieira P" first="Paulo Cezar" last="Vieira">Paulo Cezar Vieira</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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